In recent years, researchers have been hard at work developing targeted tools to sequence and genetically modify bacterial genomes, contributing to a burgeoning field of research. A Hidden World of ...

All specific primers must be supplied by the client, but we have available free of charge a few common sequencing primers: M13(-21)F and M13(-40)R. Please note: there are several primers called: M13F, ...

A Sanger sequencing reaction is run with a single primer. The following table lists the recommended amount of DNA template and primer for optimal Sanger sequencing results. Note: for plasmid DNA the ...

This technique involved insertion of the gene of interest into living bacterial cells ... During these thermal cycles, DNA primers bind to the target DNA sequence, enabling DNA polymerases ...

The inserts were sequenced using primers matching the vector sequence flanking the genomic DNA insert, and overlapping shotgun clones were used to generate a DNA sequence spanning the entire BAC ...

Rather, this method harnesses the dual catalytic activities of Vaccinia t The researcher first PCR-amplifies the gene or gene segment of interest using a proofreading polymerase and primers that ...

Gain precise, in-depth insights into microbial genomes with Illumina’s comprehensive Methods for Microbial Single-Genome Sequencing guide.

Single-nucleotide substitution in a DNA sequence that encodes a protein may ... give us insights into the evolutionary pressures on bacterial pathogens.

Amplicon-based sequencing approaches have been widely adopted for routine microbiology applications, including targeted 16S rRNA for bacterial taxonomic classification and tiled multiplex PCR for ...

Antibiotic resistance is a global concern that threatens our ability to prevent and treat bacterial infections ... This method uses RNA guides called primers to identify the specific DNA sequences ...