In recent years, researchers have been hard at work developing targeted tools to sequence and genetically modify bacterial genomes, contributing to a burgeoning field of research. A Hidden World of ...

A Sanger sequencing reaction is run with a single primer. The following table lists the recommended amount of DNA template and primer for optimal Sanger sequencing results. Note: for plasmid DNA the ...

All specific primers must be supplied by the client, but we have available free of charge a few common sequencing primers: M13(-21)F and M13(-40)R. Please note: there are several primers called: M13F, ...

This technique involved insertion of the gene of interest into living bacterial cells ... During these thermal cycles, DNA primers bind to the target DNA sequence, enabling DNA polymerases ...

Gain precise, in-depth insights into microbial genomes with Illumina’s comprehensive Methods for Microbial Single-Genome Sequencing guide.

Amplicon-based sequencing approaches have been widely adopted for routine microbiology applications, including targeted 16S rRNA for bacterial taxonomic classification and tiled multiplex PCR for ...

That’s kind of what [Ciro] did. Wanting to determine the bacteria in a water sample led to using relatively inexpensive DNA sequencing hardware to look at the DNA present in the samples.

Antibiotic resistance is a global concern that threatens our ability to prevent and treat bacterial infections ... This method uses RNA guides called primers to identify the specific DNA sequences ...