Pioneering research led by the University of Bristol has repurposed a gene-editing tool to help shed light on the true ...

An international team of microbiologists from the Medical University of Graz, the DSMZ—German Collection of Microorganisms ...

After 72 h, the cells were collected and used for genotyping by Sanger sequencing. NSUN6-depleted cell lines were generated in the same way. The primers used for genotyping and single guide RNA ...

To identify bacteria, the hypervariable region V3–V4 of the 16S rRNA gene was amplified using universal primers 341F/806R, following previous protocols (Ran et al., 2023). High-throughput sequencing ...

Rather, this method harnesses the dual catalytic activities of Vaccinia t The researcher first PCR-amplifies the gene or gene segment of interest using a proofreading polymerase and primers that ...

BIGSdb is software designed to store and analyse sequence data for bacterial isolates. Any number of sequences can be linked to isolate records - these can be small contigs assembled from dideoxy ...

Bacteria and their metabolites exhibit remarkable diversity ... This outcome indicated that 16S sequencing using the 27F/1492R primer pair was insufficient for definitive species-level identification ...

Genome sequencing is an advanced technique that deciphers the complete genetic material of TB-causing bacteria. By studying genetic mutations, scientists can identify drug-resistant strains and ...

In order to optimize the sequencing reaction we need information about template and primer concentrations, size of the template DNA and annealing temperature of the primer. Our requirements for ...