In recent years, researchers have been hard at work developing targeted tools to sequence and genetically modify bacterial genomes, contributing to a burgeoning field of research. A Hidden World of ...

An infectious diseases detection platform developed by University of Pittsburgh scientists working with UPMC infection preventionists proved over a two-year trial that it stops outbreaks, saves lives ...

Rather, this method harnesses the dual catalytic activities of Vaccinia t The researcher first PCR-amplifies the gene or gene segment of interest using a proofreading polymerase and primers that ...

All specific primers must be supplied by the client, but we have available free of charge a few common sequencing primers: M13(-21)F and M13(-40)R. Please note: there are several primers called: M13F, ...

Antibiotic resistance is a global concern that threatens our ability to prevent and treat bacterial infections ... This ...

Moreover, cultivating harmful bacteria poses major risks to the scientists handling them and requires specialized equipment and strict safety protocols. Modern sequencing technologies offer ...

A Sanger sequencing reaction is run with a single primer. The following table lists the recommended amount of DNA template and primer for optimal Sanger sequencing results. Note: for plasmid DNA the ...

Amplicon-based sequencing approaches have been widely adopted for routine microbiology applications, including targeted 16S rRNA for bacterial taxonomic classification and tiled multiplex PCR for ...